Kelimpahan Bakteri Endofit Lamun Enhalus sp. di Kuta Lombok Tengah dan Halodule sp. di Sekotong Lombok Barat
DOI:
https://doi.org/10.35721/jakiyah.v11iss1pp%25pKeywords:
Endophytic Bacteria, Enhalus sp, Halodule sp, Total Plate CountAbstract
atar Belakang: Ekosistem pesisir memiliki keanekaragaman hayati mikroorganisme yang tinggi, namun
eksplorasi bakteri endofit pada tumbuhan pesisir seperti lamun masih relatif terbatas. Kondisi lingkungan pesisir
yang memiliki berbagai cekaman ekologis berpotensi membentuk komunitas bakteri endofit yang khas, sehingga
penelitian mengenai kelimpahan bakteri endofit pada lamun menjadi penting untuk dilakukan.
Tujuan: Penelitian ini bertujuan untuk mengetahui kelimpahan serta karakteristik bakteri endofit yang diisolasi
dari lamun Enhalus sp. dan Halodule sp. yang berasal dari dua lokasi pesisir yang berbeda, yaitu Kuta Lombok
Tengah dan Sekotong Lombok Barat.
Metode: This research is a descriptive research. Penelitian ini merupakan penelitian deskriptif. Metode penelitian
yang digunakan meliputi isolasi bakteri endofit menggunakan metode Total Plate Count dengan teknik spread
plate pada media Nutrient Agar, dilanjutkan dengan pemurnian isolat serta karakterisasi makroskopis dan
mikroskopis.
Hasil penelitian: Hasil penelitian menunjukkan bahwa kelimpahan bakteri endofit berbeda antara kedua jenis
lamun, dengan nilai kelimpahan tertinggi diperoleh dari lamun Enhalus sp. (2,1x103 ) dibandingkan Halodule sp.
(1,8x103 ). Karakterisasi koloni menunjukkan variasi morfologi koloni yang meliputi bentuk, warna, tepian, dan
elevasi koloni, sedangkan hasil pengamatan mikroskopis menunjukkan adanya bakteri Gram positif dengan bentuk
sel dominan berupa basil dan kokus. Perbedaan kelimpahan bakteri endofit pada kedua jenis lamun diduga
dipengaruhi oleh karakteristik habitat serta kondisi lingkungan perairan di masing-masing lokasi.
Simpulan: Kelimpahan bakteri endofit Enhalus sp yang diisolasi dari lamun Pantai Kuta lebih tinggi
dibandingankan dengan bakteri endofit Halodule sp yang diisolasi dari lamun Pantai Sekotong.
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